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The Repertoire of Newly Developing Regulatory T Cells in the Type I Diabetes-Prone NOD Mouse is Very Diverse

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posted on 25.05.2021, 23:20 by Ariel Galindo-Albarrán, Sarah Castan, Jérémy C. Santamaria, Olivier P. Joffre, Bart Haegeman, Paola Romagnoli, Joost P.M. van Meerwijk
Regulatory T lymphocytes expressing the forkhead/winged helix transcription factor Foxp3 (Treg) play a vital role in the protection of the organism from autoimmune disease and other immunopathologies. The antigen-specificity of Treg plays an important role in their in vivo activity. We therefore assessed the diversity of the T cell receptors for antigen (TCR) expressed by Treg newly developed in the thymus of autoimmune type I diabetes-prone NOD mice and compared it to the control mouse strain C57BL/6. Our results demonstrate that usage of the TCRa and TCRb variable (V) and joining (J) segments, length of the complementarity determining region (CDR) 3, and the diversity of the TCRa and TCRb chains are comparable between NOD and C57BL/6 mice. Genetic defects affecting the diversity of the TCR expressed by newly developed Treg therefore do not appear to be involved in the etiology of type I diabetes in the NOD mouse.

Funding

This work was financially supported by the Fondation pour la Recherche Médicale (to JvM, DEQ20160334920; to JCS, FDT201904008280); the IdEx Toulouse (to PR); the Région Midi Pyrénées (to JvM, 15/06/12.05); the Agence Nationale pour la Recherche (to PR, ANR-16-CE15-0015-01).

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