posted on 2021-07-27, 17:52authored byXiaojing Lin, Yunqi Zhang, Xiaoling He, Yan Chen, Nan Chen, Juncheng Liu, Miaoran Wang, Yue Li, Hong Yang, Lei Fan, Yi Hou, Jibin Li, Chaodong Wu, Hongbo Qi, Hua Zhang, Xiaoqiu Xiao
Choline metabolite Trimethylamine N-oxide
(TMAO) has been recognized as a risk factor of gestational diabetes mellitus
(GDM), but its exact role in GDM has not been reported. In this study, we focused on the placenta development
to reveal the role of TMAO in GDM. We found the TMAO levels in
peripheral and cord plasma were increased in women with GDM, and TMAO levels were positively
correlated with newborn weight and placental thickness. Neutrophil extracellular traps (NETs) in the peripheral and
cord plasma and the myeloperoxidase
expression in the placenta of women with GDM also increased. NETs could
inhibit the proliferation, migration, invasion and angiogenesis of HTR-8/Svneo
cells. However, TMAO could not only inhibit the
formation of NETs, but also enhance the biological function of HTR-8/Svneo
cells. By inducing GDM models in NETs deficient PAD4-/- and
wild-type mice, the placental weight of PAD4-/- mice increased significantly.
TMAO feeding
also inhibited the formation of NETs and further increased the weight of
the placenta and fetuses, and this increase did not affect the placental
structure. Our data indicated that higher TMAO levels and the formation of
abnormal NETs were associated with GDM. TMAO could not only promote the
development of the placenta and fetuses, but also inhibit the formation of
NETs.
Funding
This work was supported by the National Natural Science Foundation of China (82071734 and 81871222 to XX), the Fundamental Science and Advanced Technology Research of Chongqing (CSTC2015jcyjB 0146, to XX) and Basic Research and Frontier Exploration of Science and Technology Commission by Chongqing Municipality (CSTC2018jcyjAX0788, to YC).