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Plasma exosomal microRNAs associated with metabolism as early predictor of gestational diabetes mellitus

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posted on 04.08.2022, 18:33 authored by Zhixin Ye, Songzi Wang, Xiaoqing Huang, Peisong Chen, Langhui Deng, Shiqi Li, Suiwen Lin, Zilian Wang, Bin Liu

To date, the microRNA (miRNA) expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small RNA sequencing in 12 GDM and 12 normal glucose tolerance (NGT) pregnant women and validated in 102 GDM and 101 NGT pregnant women. A total of 22 exosomal miRNAs were found and five of them were verified by qRT-PCR. Exosmal miR-423-5p was upregulated, while miR-122-5p, miR-148a-3p, miR-192-5p, and miR-99a-5p were downregulated in women whose pregnancy is complicated by GDM. IGF1R and GYS1 as target genes of miR-423-5p, and G6PC3 and FDFT1 as target genes of miR-122-5p, were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs showed an AUC of 0.82 (95%CI, 0.73~0.91) in early prediction of GDM. Our study demonstrated that dysregulated exosomal miRNAs in plasma from GDM pregnant women might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM. 

Funding

This work was supported by the National Natural Science Foundation of China (No. 81771602, 81300493 to Bin Liu and 81701378 to Zilian Wang), Natural Science Foundation of Guangdong Province (No. 2017A030313826, to Bin Liu, and No.2017A020214014, to Zilian Wang), Sun Yat-Sen University Clinical Research 5010 Program (No.2016014, to Bin Liu, and No. 201204, to Zilian Wang), Fundamental Research Funds for the Central Universities (No. 16ykpy19, to Bin Liu), National Key Research and Development Program of China (No. 2021YFC2700700, Sub-grant to Zilian Wang). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

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