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Pancreatic β-cell specific deletion of VPS41 causes diabetes due to defects in insulin secretion
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posted on 2020-11-10, 16:42 authored by Ada AdminAda Admin, Christian H. Burns, Belinda Yau, Anjelica Rodriguez, Jenna Triplett, Drew Maslar, You Sun An, Reini E.N. van der Welle, Ross G. Kossina, Max R. Fisher, Gregory W. Strout, Peter O. Bayguinov, Tineke Veenendaal, David Chitayat, James A.J. Fitzpatrick, Judith Klumperman, Melkam A. Kebede, Cedric S. AsensioInsulin secretory granules (SGs) mediate the regulated
secretion of insulin, which is essential for glucose homeostasis. The basic
machinery responsible for this regulated exocytosis consists of specific
proteins present both at the plasma membrane and on insulin SGs. The protein
composition of insulin SGs thus dictates their release properties, yet the
mechanisms controlling insulin SG formation, which determines this molecular
composition, remain poorly understood. VPS41, a component of the endo-lysosomal
tethering HOPS complex, was recently identified as a cytosolic factor involved in the formation of neuroendocrine and neuronal granules. We
now find that VPS41 is required for insulin SG biogenesis and regulated insulin
secretion. Loss of VPS41 in pancreatic b-cells leads to a reduction
in insulin SG number, changes in their transmembrane protein composition, and
defects in granule regulated exocytosis. Exploring a human point mutation,
identified in patients with neurological but no endocrine defects, we show that
the effect on SG formation is independent of HOPS complex formation. Finally,
we report that mice with a deletion of VPS41 specifically in β-cells develop diabetes due to severe
depletion of insulin SG content and a defect in insulin secretion. In sum, our data demonstrate that VPS41 contributes to
glucose homeostasis and metabolism.