American Diabetes Association
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Measurement of Serum N-Glycans in the Assessment of Early Vascular Aging (Arterial Stiffness) in Adults with Type 1 Diabetes

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posted on 2022-08-19, 11:56 authored by Gemma Llauradó, Núria Amigó, Rocío Fuertes-Martín, Ana Romero, Albert Cano, Lara Albert, Olga Giménez-Palop, Eugenio Berlanga, Sonia Fernández-Veledo, Xavier Correig, Joan Vendrell, José-Miguel González-Clemente


OBJECTIVE: Vascular aging (arterial stiffness, AS) is an inflammation-linked process that predicts macro- and microvascular complications in adults with type 1 diabetes (T1D). We evaluated the utility of measuring the inflammation-linked N-glycans GlycA and GlycB to assess vascular aging in adults with T1D.

RESEARCH DESIGN AND METHODS: Eighty-four adults with T1D (>10-year duration without cardiovascular events) and 68 healthy controls were evaluated for clinical characteristics (including microvascular complications in patients with T1D), aortic pulse wave velocity (aPWV, surrogate measure of AS), and serum GlycA and GycB (peak area [concentration] and height/width [H/W] ratio) using 1H-NMR spectroscopy.

RESULTS: Patients with T1D had higher median (IQR) values than healthy controls for (P<0.001 for all comparisons): aPWV (7.9 [6.9–9.1] vs. 6.1 [5.5–6.7] m/s), GlycA (850.4 [781.3–916.1] vs. 652.4 [581.5–727.1] µmoL) and GlycB (386.1 [353.2–426.3] vs. 310.0 [280.5–331.9] µmol/L), and H/W ratios for GlycA (16.5 [14.9–18.1] vs. 15.0 [13.7–16.7]) and GlycB (5.0 [4.6–5.5] vs. 4.0 [3.4–4.3]). Moreover, aPWV correlated (P<0.001 for all correlations) with GlycA (r=0.550) and GlycB (r=0.423) concentrations and with H/W ratios of GlycA (r=0.453) and GlycB (r=0.510). Adjusting for potential confounders, GlycA concentration (beta=0.212, P<0.001) and the H/W ratios of GlycA (beta=0.150, P=0.009) and GlycB (beta=0.155, P=0.011) remained independently associated with aPWV. C-statistics for detecting individuals with aPWV >10 m/s were 0.866 (95%CI, 0.794–0.937) for GlycA levels and 0.862 (95%CI, 0.780–0.943) for GlycB H/W ratio. 

CONCLUSIONS: Measurement of serum GlycA/B may have utility in assessing vascular aging in adults with T1D of >10-year duration and no previous cardiovascular events. 


Financial support was provided through a grant from the Fondo de Investigación Sanitaria (FIS) PI15/00567 as part of the National R+D+I (2008–2011) and was co-financed by the Instituto de Salud Carlos III - General Evaluation Branch (Spanish Ministry of Economy and Competitiveness) and the European Regional Development Fund (ERDF).