Supplemental_Figures-R1.pdf (1.5 MB)

Maternal Obesity and Western-style Diet Impair Fetal and Juvenile Offspring Skeletal Muscle Insulin-Stimulated Glucose Transport in Nonhuman Primates

Download (1.5 MB)
posted on 30.04.2020 by Ada Admin, William Campodonico-Burnett, Byron Hetrick, Stephanie R. Wesolowski, Simon Schenk, Diana L. Takahashi, Tyler A. Dean, Elinor L. Sullivan, Paul Kievit, Maureen Gannon, Kjersti Aagaard, Jacob E. Friedman, Carrie E. McCurdy
Infants born to mothers with obesity have a greater risk for childhood obesity and metabolic diseases; however, the underlying biological mechanisms remain poorly understood. We used a Japanese macaque model to investigate whether maternal obesity combined with a western-style diet (WSD) impairs offspring muscle insulin action. Adult females were fed a control or WSD prior to and during pregnancy through lactation, and offspring subsequently weaned to a control or WSD. Muscle glucose uptake and signaling were measured ex vivo in fetal (n=5-8/group) and juvenile offspring (n=8/group). In vivo signaling was evaluated after an insulin bolus just prior to weaning (n=4-5/group). Maternal WSD reduced insulin-stimulated glucose uptake and impaired insulin signaling at the level of Akt phosphorylation in fetal muscle. In juvenile offspring, insulin-stimulated glucose uptake was similarly reduced by both maternal and post-weaning WSD and corresponded to modest reductions in insulin-stimulated Akt phosphorylation relative to controls. We conclude that maternal WSD leads to a persistent decrease in offspring muscle insulin-stimulated glucose uptake even in the absence of increased offspring adiposity or markers of systemic insulin resistance. Switching offspring to a healthy diet did not reverse the effects of maternal WSD on muscle insulin action suggesting earlier interventions may be warranted.


This research was supported by grants K12 HD057022 (C.E.M.), R24 DK090964 (J.E.F., K.M.A.), and R01 DK089201 (K.M.A) from the National Institute of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.