American Diabetes Association
2022-05-31_-_Supplementary_-_Vitreous_RBP3.pdf (523.41 kB)

Elevated Retinol Binding Protein 3 Concentrations Are Associated with Decreased Vitreous Inflammatory Cytokines, VEGF and Progression of Diabetic Retinopathy

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posted on 2022-07-19, 13:00 authored by Ward Fickweiler, Hyunseok Park, Kyoungmin Park, Margalit G. Mitzner, Tanvi Chokshi, Tahani Boumenna, John Gautier, Yumi Zaitsu, I-Hsien Wu, Jerry Cavallerano, Lloyd P. Aiello, Jennifer K. Sun, George L. King


OBJECTIVE: To correlate inflammatory cytokines and vascular endothelial growth factor (VEGF) in vitreous and plasma with vitreous Retinol Binding Protein 3 (RBP3), diabetic retinopathy (DR) severity, and DR worsening in population with type 1 and type 2 diabetes.
RESEARCH DESIGN AND METHODS: RBP3, VEGF and inflammatory cytokines were measured in plasma and vitreous samples (n=205) from subjects of the Joslin Medalist Study and Beetham Eye Institute.
RESULTS: Higher vitreous RBP3 concentrations were associated with less severe DR (p<0.0001), and reduced risk of developing proliferative DR (PDR, p<0.0001). Higher RBP3 correlated with increased photoreceptor segment thickness and lower vitreous IL-12, TNF-a and TNF-b  (p<0.05). PDR was associated with lower vitreous IFN-γ and IL-10, and higher VEGF, IL-6, and IL-15 (p<0.05), but was not associated with their plasma concentrations.
CONCLUSIONS: Higher vitreous RBP3 concentrations are associated with less severe DR and slower rates of progression to PDR, supporting its potential as a biomarker and  therapeutic agent for preventing DR worsening, possibly by lowering retinal VEGF and inflammatory cytokines.


Support were from: National Eye Institute (R01EYE26080-01), ADA (7-21-PDF-022, to W.F.), the National Institute of Diabetes and Digestive and Kidney Diseases and National Institutes of Health (DP3-DK-094333-01); JDRF (17-2013-310), the Dianne Nunnally Hoppes Fund, the Beatson Fund, and many Medalists.