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Distinct Characteristics between Perivascular and Subcutaneous Adipose Derived Stem Cells

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posted on 09.11.2021, 17:55 by Yao Xie, Yongli Ji, Yunrui Lu, Yuankun Ma, Hui Ni, Jian Shen, Hong Ma, Chunna Jin, Yuwen Chen, Yan Lin, Meixiang Xiang
Adipose derived stem cells (ADSCs) can differentiate into vascular lineages and participate in vascular remodeling. Perivascular ADSCs (PV-ADSCs) draw attention due to their unique location. The heterogeneity of subcutaneous (SUB-) and abdominal ADSCs were well addressed, but PV-ADSCs’ heterogeneity hasn’t been investigated. In the present study, we applied single-cell analysis to compare SUB-ADSCs and PV-ADSCs respectively regarding their subpopulations, functions, and cell fates. We uncovered 4 subpopulations of PV-ADSCs including Dpp4+, Col4a2+/Icam1+, Clec11a+/Cpe+ and Sult1e1+ cells, among which Clec11a+ subpopulation potentially participated in and regulated the PV-ADSCs differentiation towards a smooth muscle cell (SMC) phenotype. The present study revealed the distinct characteristics between PV-ADSCs and SUB-ADSCs.

Funding

This research was supported by the grant from The National Natural Science Foundation of China (NSFC) [grant number: 81800427(Yao Xie), 81870203 (Meixiang Xiang)].

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