posted on 2021-06-23, 15:22authored byPeng Yi, Noelle Morrow
genome editing system has been one of the greatest scientific discoveries in
the last decade. The highly efficient and precise editing ability of this
technology is of great therapeutic value and benefits the basic sciences as an
advantageous research tool. In recent years, forward genetic screens utilizing
CRISPR technology have been widely adopted, with genome-wide or pathway-focused
screens leading to important and novel discoveries. CRISPR screens have been
used primarily in cancer biology, virology and basic cell biology; but they
have rarely been applied to diabetes research. A potential reason for this is
that diabetes related research can be more complicated, often involving cross-talk
between multiple organs or cell types. Nevertheless, many questions can still
be reduced to the study of a single cell type if assays are carefully designed.
Here we review the application of CRISPR screen technology and provide
perspective on how it can be used in diabetes research.